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目的探讨富含半胱氨酸的酸性分泌蛋白(secreted protein acidic and rich in cysteine,SPARC)表达水平与宫颈病变进程的关系及其临床意义。方法收集宫颈炎、宫颈内上皮瘤样病变(CIN)及宫颈鳞癌患者的石蜡包埋组织标本107例,采用免疫组织化学SP法检测SPARC蛋白表达水平。结果 SPARC蛋白在宫颈间质细胞内表达,在宫颈炎的表达水平很低(23%),随着CINⅠ~Ⅱ和CINⅢ病理进程呈梯度性增高(35%和61%),在所有的宫颈癌标本中可以检出SPARC蛋白(100%),宫颈炎组和CINⅠ~Ⅱ组之间的表达差异无统计学意义,其他各组之间的表达差异均有统计学意义(P<0.05)。宫颈鳞癌中SPARC蛋白的表达差异与宫颈鳞癌的病理分化程度之间无统计学意义(P>0.05)。结论 SPARC蛋白表达水平的上调可能是宫颈病变进程的分子标志,这为揭示宫颈癌发病机制及建立临床评估体系提供了重要依据。
Objective To investigate the relationship between the expression level of cysteine-rich acidic secreted protein (SPARC) and the progression of cervical lesions and its clinical significance. Methods 107 cases of cervicitis, cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma were collected. SPARC protein expression was detected by immunohistochemical SP method. Results The expression of SPARC protein in cervical stromal cells was very low (23%). With the pathological progression of CINⅠ ~ Ⅱ and CINⅢ progressively increased (35% and 61%), all the cervical cancer SPARC protein (100%) could be detected in the specimens. There was no significant difference between cervicitis group and CINⅠ ~ Ⅱ group (P <0.05). Cervical squamous cell carcinoma SPARC protein expression and cervical squamous cell carcinoma differentiation between no significant difference (P> 0.05). Conclusion Upregulation of SPARC protein expression may be a molecular marker of cervical lesions, which provides an important basis for revealing the pathogenesis of cervical cancer and establishing a clinical evaluation system.