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花生是重要的豆科经济作物,能与慢生根瘤菌属的根瘤菌共生固氮,辽宁省的花生种植较广泛,但是对该地区花生根瘤菌的研究并不全面。采用16S r DNA和nif H基因的序列分析方法从遗传学角度对17株分离自辽宁省不同地区的花生根瘤菌的特性进行研究。根据16S r DNA基因的序列分析将所有菌株分为4个类群,而nif H基因的序列分析方法将供试菌株分成两大类群,2种方法的分类结果大体一致,测序结果表明所有菌株都属于慢生根瘤菌属。说明辽宁省花生慢生根瘤菌的分布可能与该地的地理环境存在一定的相关性。
Peanut is an important leguminous cash crop and can coexist with Rhizobium of Brachyspira. The peanut cultivation in Liaoning Province is more extensive, but the research on the peanut rhizobia in this area is not comprehensive. Sequence analysis of 16S r DNA and nifH genes was used to study the characteristics of 17 strains of peanut rhizobia isolated from different areas of Liaoning Province from the perspective of genetics. All the strains were divided into four groups according to the sequence analysis of 16S r DNA gene, and the sequence analysis method of nif H gene divided the tested strains into two groups. The classification results of the two methods were roughly the same, and the sequencing results showed that all the strains belonged to Bradyrhizobium. This indicated that the distribution of Bradyrhizobia in peanut in Liaoning Province may have some correlation with the geographical environment of this area.