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在逆转录病毒介导下将ＴＮＦ基因转染入小鼠ＬＡＫ细胞中，采用Ｇ４１８抗性筛选和有限稀释法，将ＬＡＫ细胞克隆化，并筛选出３株分泌不同水平ＴＮＦ的ＬＡＫ细胞克隆。结果表明，这３株ＬＡＫ细胞克隆分泌的ＴＮＦ水平、增殖能力和杀伤活性均显著高于对照组ＬＡＫ细胞。而高分泌株的ＬＡＫ细胞克隆，其增殖能力和杀伤活性最高；中分泌株次之；低分泌株相对较低。抗ＴＮＦ单抗可显著抑制此３株ＬＡＫ细胞体外杀伤活性，表明其杀伤活性升高是由基因转染后所表达的ＴＮＦ介导的，所表达ＴＮＦ水平越高，其增殖能力和杀伤活性越高。
The TNF gene was transfected into mouse LAK cells by retroviral vector. LAK cells were cloned by G418 resistance screening and limited dilution method. Three LAK cell clones secreting different levels of TNF were screened. The results showed that the levels of TNF, proliferation and cytotoxicity secreted by these three LAK cell clones were significantly higher than that of the control LAK cells. The LAK cell clone of the highly secreted strain had the highest proliferative and cytotoxic activity, followed by the middle secreted strain and the lower secreted strain. Anti-TNF monoclonal antibody could significantly inhibit the killing activity of these three LAK cells in vitro, indicating that the increase of cytotoxic activity was mediated by TNF expressed after gene transfection, and the higher the level of TNF expressed, the more its proliferative ability and cytotoxic activity high.