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目的采用LC-MS/MS方法测定16种含乌头属中药制剂中8种乌头类生物碱含量。方法以90%甲醇-水(水相含0.1%甲酸和2.5 mmol/L醋酸铵水溶液)为流动相,Agilent Zorbax SB-C18色谱柱分离,采用ESI(+),MRM监测方法,对16种市售含乌头属中药制剂中苯甲酰乌头原碱、苯甲酰次乌头原碱、苯甲酰新乌头原碱,乌头碱、次乌头碱、新乌头碱,新乌头原碱、乌头原碱进行含量测定。结果上述8种生物碱的线性关系良好,精密度、重复性良好,加样回收率为95.3%~102.3%,不同含乌头属中药制剂中8种生物碱成分的含量差别较大。结论该方法快速、灵敏,可靠,可应用于对乌头属中药制剂的质量控制。
Objective To determine the content of eight kinds of aconite in 16 kinds of Aconitum by LC-MS / MS. Methods The chromatographic separation was performed on an Agilent Zorbax SB-C18 column with 90% methanol-water (0.1% formic acid in water and 2.5 mmol / L ammonium acetate in water) as mobile phase. Sale aconitine containing aconitine aconitine base, benzoyl aconitine base, benzoyl mesaconitine, aconitine, hypaconitine, mesaconitine, Xinwu Head original alkali, aconitine base content determination. Results The above eight alkaloids had good linearity, good precision and reproducibility. The recoveries of the eight alkaloids were 95.3% -102.3%. The contents of eight alkaloids in different Aconitum preparations differed greatly. Conclusion The method is rapid, sensitive and reliable and can be applied to the quality control of Aconitum preparations.