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利用胶体金标记的经亲和层析纯化的氯嘧磺隆单克隆抗体,氯嘧磺隆-牛血清白蛋白,羊抗鼠IgG制备了氯嘧磺隆胶体金免疫检测试纸条。该试纸条的最低检测极限为100μg/L,检测时间10 m in。8个土壤样品分别添加0~2000μg/L的氯嘧磺隆标样,用0.15 mol/L NaHCO3提取2 h后用气相色谱和试纸条进行检测,如果以100μg/L作为阳性与阴性样品的区分标准,气相色谱和试纸条检测结果完全一致。所制备的胶体金免疫检测试纸条可以用于土壤中氯嘧磺隆残留的筛选。
The colloidal gold immunochromatographic strip was prepared by colloidal gold labeled affinity chromatography purified monoclonal antibody against chlorimuron-ethyl, chlorimuron-bovine serum albumin and goat anti-mouse IgG. The test strip minimum detection limit of 100μg / L, detection time 10 m in. 8 soil samples were added 0 ~ 2000μg / L of chlorosulfuron standard samples with 0.15 mol / L NaHCO3 extracted 2 h after gas chromatography and test strips were detected, if the 100μg / L as positive and negative samples Distinguish between standards, gas chromatography and test strip test results are exactly the same. The prepared colloidal gold immunoassay strip can be used for screening of residues of chlorimuron-ethyl in the soil.