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为探讨不同Ｘ线处理对脑胶质瘤细胞增殖的影响，选用人脑星形细胞瘤ＳＷＯ－３８系，采用ＭＴＴ法观察Ｘ线对胶质瘤细胞生长的影响，ＡＢＣ法检测增殖细胞核抗原（ＰＣＮＡ）基因表达的变化。结果显示：①不同剂量（５，１０，１５，２０，２５Ｇｙ）Ｘ线对胶质瘤细胞生长具有明显的抑制作用，生长曲线低平，照射后４８ｈ的抑制率分别为３３％、４７％、５１％、５８％和６６％；②不同剂量率（８０，１６０，２４０，３２０，４００ｃＧｙ／ｍｉｎ）Ｘ线照射后４８ｈ的抑制率分别为５８％、５４％、４７％、２５％和３０％；③超分割照射后４８ｈ的抑制率为６２％，而单次照射组为５０％；④不同剂量（５，１０，１５，２０，２５Ｇｙ）Ｘ线照射后ＰＣＮＡ－标记率（ＬＩ）分别为９１％，８７％，８１％，７８％，６９％，ＰＣＮＡ－ＬＩ与存活细胞ＯＤ值呈显著性正相关关系（Ｐ＜０．０５）。说明①ＳＷＯ细胞生长曲线、抑制率与Ｘ线剂量、剂量率和照射方式之间密切相关；②ＰＣＮＡ－ＬＩ与存活细胞ＯＤ值呈正相关关系，ＰＣＮＡ是胶质瘤细胞增殖的标志之一。
In order to investigate the effect of different X-ray treatment on the proliferation of glioma cells, the human astrocytoma SWO-38 line was selected. The effect of X-ray on the growth of glioma cells was observed by MTT method. The proliferative cell nuclear antigen PCNA) gene expression changes. The results showed that: (1) X-ray at different doses (5, 10, 15, 20, 25 Gy) significantly inhibited the growth of glioma cells and the growth curve was leveled with the inhibition rates of 33%, 47% 51%, 58%, and 66% respectively; ②The inhibitory rates at different dose rates (80,160,240,320,400cGy / min) 48h after radiotherapy were 58%, 54%, 47%, 25% and 30% ; ③ The inhibition rate of 48h after superfractionated irradiation was 62%, while that of single irradiation group was 50%. ④ The PCNA-labeling rate (LI) after X-ray irradiation at different doses (5,10,15,20,25Gy) 91%, 87%, 81%, 78%, 69%. There was a significant positive correlation between PCNA-LI and surviving cell OD (P <0.05). There was a positive correlation between PCNA-LI and OD value of viable cells, and PCNA was one of the markers of glioma cell proliferation.