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目的探讨白细胞介素-5(IL-5)I、L-17在不同亚型中重度儿童哮喘发病中的作用。方法以酶联免疫吸附法(ELISA)检测45例不同亚型中重度哮喘患儿及20例正常儿童外周血单个核细胞(PBMC)在CD3单克隆抗体(0.2mg/L)及CD28单克隆抗体(1mg/L)刺激下分泌的IL-5I、L-17水平,了解IL-5I、L-17与儿童哮喘病情及气道炎症的关系。结果嗜酸细胞型哮喘(EA)组患儿PBMC表达IL-5水平(79.75±13.49)pg/ml显著高于非嗜酸细胞型哮喘(NEA)组(69.73±9.17)pg/ml及正常对照组(51.30±14.33)pg/ml(P<0.01),NEA组患儿PBMC表达IL-17水平(5684.1±884.74)pg/ml显著高于EA组(4938.1±465.58)pg/ml及正常对照组(2685.0±1081.03)pg/ml(P<0.01),慢性持续组IL-5、IL-17水平显著高于临床缓解组(P均<0.05)。结论中重度哮喘患儿外周血单个核细胞IL-5I、L-17表达水平升高,并与临床分期相关I,L-5在EA中的作用较NEA更为突出I,L-17在NEA中的作用较EA更为突出。
Objective To investigate the role of interleukin-5 (IL-5) I and L-17 in the pathogenesis of asthma in children with different subtypes. Methods Serum levels of CD3 monoclonal antibody (0.2 mg / L) and CD28 monoclonal antibody (PBMC) in peripheral blood mononuclear cells (PBMCs) of 45 children with moderate and severe asthma and 20 normal children were detected by enzyme linked immunosorbent assay (ELISA) (1mg / L) to stimulate the secretion of IL-5I, L-17 levels to understand the IL-5I, L-17 and childhood asthma and airway inflammation. Results The level of IL-5 in PBMC of eosinophilic asthmatic group (79.75 ± 13.49 pg / ml) was significantly higher than that of non-eosinophilic asthmatic group (69.73 ± 9.17 pg / ml) and normal controls The level of IL-17 in PBMC of NEA group (5684.1 ± 884.74) pg / ml was significantly higher than that of EA group (4938.1 ± 465.58) pg / ml and the control group (51.30 ± 14.33) pg / ml (2685.0 ± 1081.03) pg / ml (P <0.01). The levels of IL-5 and IL-17 in chronic persistent group were significantly higher than those in clinical remission group (all P <0.05). Conclusion The expression of IL-5I and L-17 in peripheral blood mononuclear cells of moderate-severe asthma patients is increased, and the role of I and L-5 in EA is more prominent than NEA in clinical staging. The expression of L-17 in NEA The role of EA more prominent.