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目的探讨二氯甲烷(DCM)染毒对小鼠血清中肝功能及肝组织病理的影响。方法将昆明种雄性小鼠随机分为高、中、低剂量染毒组(染毒剂量分别为12 900、6 400、1 300mg/m3)和对照组(0mg/m3),每组6只。将小鼠成笼放入动式染毒室内,每天染毒2h,连续染毒30d后,检测血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、总抗氧化能力(T-AOC)水平;取小鼠肝脏作石蜡切片,HE染色观察肝脏组织病理学变化。结果与对照组比较,各染毒组ALT、AST、ALP水平均有升高,差异均有统计学意义(P<0.05);与对照组比较,各染毒组SOD、MDA含量均有升高(P<0.05),GSH含量、T-AOC水平随染毒剂量的增加呈下降趋势(P<0.05);组织病理切片显示小鼠肝脏出现炎症反应。结论 DCM吸入染毒对小鼠的肝脏功能有一定的影响,并出现炎症性病理变化。
Objective To investigate the effects of methylene chloride (DCM) on the serum levels of liver function and liver pathology in mice. Methods Kunming male mice were randomly divided into high, medium and low dose groups (12 900, 6 400 and 1 300 mg / m3, respectively) and control group (0 mg / m3), 6 in each group. The mice were housed in a mobile poisoning room and exposed to a daily dose of 2 hours for 30 days. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) ), Malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and total antioxidant capacity (T-AOC) were measured. The liver of the mice was made into paraffin sections, Pathological changes. Results Compared with the control group, the levels of ALT, AST and ALP were increased in all treatment groups, the differences were statistically significant (P <0.05). Compared with the control group, the contents of SOD and MDA increased (P <0.05). The contents of GSH and T-AOC decreased with the increase of dose (P <0.05). The histopathological examination showed inflammatory reaction in the liver of mice. Conclusion Inhalation of DCM has some effects on the liver function of mice and inflammatory pathological changes.