论文部分内容阅读
本文应用同位素示踪和放射自显影技术,观察了新鲜人胚胎22例视网膜神经元体外摄取和释放~3H-甘氨酸和~3H-天门冬氨酸功能的出现。结果表明,14～15周人胚胎视网膜已具有摄取和K~+刺激、Ca~(++)依赖性释放外源性~3H-甘氨酸和~3H-天门冬氨酸的能力。摄取和~3H-甘氨酸的细胞是视网膜内核层最深层的细胞,摄取和蓄积~3H-天门冬氨酸的细胞是内核层中部和外核层的部份细胞,根据细胞形态和所在位置,我们认为这些细胞分别是发育中的无长突细胞,双极细胞和视锥细胞。此外,在~3H-天门冬氨酸的放射自显影图像中,可见视网膜的外核层、内网层和神经纤维层中有条索状银粒分布,这可能是Müller细胞被标记的结果。
In this study, isotope tracer and autoradiography were used to observe the uptake and release of ~ 3H-glycine and -3H-aspartate in vitro by 22 retinal neurons from fresh human embryos. The results showed that the human retina at 14-15 weeks had the ability of uptake and K ~ + stimulation and Ca ~ (++) dependent release of exogenous ~ 3H-glycine and -3H-aspartate. 3H-Gly-derived cells are the deepest cells in the inner nuclear layer of the retina. Cells that uptake and accumulate ~ 3H-aspartate are part of the cells in the middle and outer nuclear layers of the inner nuclear layer. Depending on the cell morphology and location, These cells are considered to be developing azoospermic cells, bipolar cells and cones. In addition, in the ~ 3H-aspartate autoradiogram, there was a barbed silver distribution in the outer nuclear layer, the inner reticular layer and the nerve fiber layer of the retina, which may be the result of Müller cells being labeled.