论文部分内容阅读
目的探讨依普利酮干预对高糖和白介素1β(IL-1β)诱导的人心脏成纤维细胞基质金属蛋白酶2(MMP-2)表达的影响。方法将人心脏成纤维细胞分别培养在正常葡萄糖浓度(正常对照组)、高浓度葡萄糖(高糖组)和等渗甘露醇(高渗对照组)的培养液中,分别添加IL-1β和(或)盐皮质激素受体阻断剂依普利酮进行干预。明胶酶法测定细胞培养上清液中MMP-2的活性,RT-PCR测定成纤维细胞MMP-2和组织金属蛋白酶抑制因子2(TIMP-2)mRNA的表达。结果与正常对照组比较,高糖组MMP-2活性和mRNA的表达显著增高(P<0.01),高渗对照组MMP-2活性和mRNA表达也增高但低于高糖组(P<0.05)。与高糖组比较,高糖+IL-1β组MMP-2活性和mRNA的表达呈现叠加现象,高糖+依普利酮组和正常对照+IL-1β+依普利酮组MMP-2活性和mRNA的表达分别显著低于高糖组和正常对照+IL-1β组(P<0.05)。结论高糖可通过高渗外的作用诱导人心脏成纤维细胞MMP-2活性增强和mRNA表达增加,IL-1β对该诱导作用具有促进作用,而依普利酮则呈现抑制效应。
Objective To investigate the effect of eplerenone on the expression of matrix metalloproteinase 2 (MMP-2) in human cardiac fibroblasts induced by high glucose and interleukin-1β (IL-1β). Methods Human cardiac fibroblasts were cultured in culture medium of normal glucose concentration (normal control group), high concentration glucose (high glucose group) and isotonic mannitol (high osmotic control group), IL-1β and Or) mineralocorticoid receptor blocker eplerenone. The activity of MMP-2 in cell culture supernatant was determined by gelatinase method. The mRNA expression of MMP-2 and TIMP-2 in fibroblasts was determined by RT-PCR. Results Compared with normal control group, MMP-2 activity and mRNA expression were significantly increased in high glucose group (P <0.01), while MMP-2 activity and mRNA expression in hyperglycemic control group were also higher than those in high glucose group (P <0.05) . Compared with high glucose group, MMP-2 activity and mRNA expression in high glucose + IL-1βgroup showed superposition, MMP-2 activity in high glucose + eplerenone group and normal control + IL-1β + eplerenone group And mRNA expression were significantly lower than the high glucose group and the normal control + IL-1β group (P <0.05). CONCLUSION: High glucose can induce MMP-2 activity and mRNA expression in human cardiac fibroblasts through hypertonic extravasation. IL-1β can promote this induction, while eplerenone can inhibit it.