Morphological characterization of amidinophenylporphyrins interacting with DNA by photo irradiation

• 作 者: Kai Wang Shuai-Hua Song Yun-Man Zheng Zao-Ying Li
• 机 构: Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology,Co
• 出 处: Chinese Chemical Letters
• 发表日期: 2013年11期
• 关键词: irradiation DNA interacting porphyrin thymus indirectly complexes morphological

5,10,15,20-Tetrakis(4-amidinophenyl)porphyrin(Por 1),its Zn complex(Por 2)and amidinophenyl bispophyrin(Por 3)interacting with calf thymus DNA were investigated by scanning electron microscopy(SEM)and the shape and size of the porphyrin–DNA complexes were observed after photo irradiation.The results showed that the shape and size of DNA had signifcant differences with blank group by virtue of the interaction between the porphyrins and ct-DNA.This suggests the morphological characterization could be used to study the interaction and judge DNA photocleavage activity indirectly through the photo irradiation of porphyrins.

Interacting with calf thymus DNA were investigated by scanning electron microscopy (SEM) and the 5,10,15,20-Tetrakis (4-amidinophenyl) porphyrin (Por 1), its Zn complex (Por 2) and amidinophenyl bispophyrin shape and size of the porphyrin-DNA complexes were observed after photo irradiation. The results showed that the shape and size of DNA had signifcant differences with blank group by virtue of the interaction between the porphyrins and ct-DNA.This suggests the morphological characterization could be used to study the interaction and judge DNA photocleavage activity indirectly through the photo irradiation of porphyrins.