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自甾体激素的二阶模式作用机理提出以来,认为甾体激素通过细胞膜以后与胞浆受体相结合,再调节基因表达;但此机理不能显示靶细胞的电位变化。有人通过糖皮质激素诱导神经元膜电位超极化的研究,论证近年一些研究者提出的甾体介导细胞膜的电位变化。这种新机理对研究神经系统具有重要的生理学意义,因为快速的膜效应是神经元功能的基础。实验以豚鼠的带内脏神经的腹腔神经节为标本,用Kreb’s溶液或考的松等溶液进行神经节表面灌流,细胞内微电极记录电位变化。实验结果表明:(1)用10~(-(?))～10~(-(?))mol/L不同浓度的半琥珀酸考的松(F)液,对豚鼠神经节表面灌流,可引起神经细胞膜电位相应的不同幅度的超极化,超极化的潜伏期少于2min;(2)在含F的低Ca~(2+)/高Mg~(2+)溶液作用下,神经元膜电位超极
Since the mechanism of the second-order mode of action of steroid hormones has been proposed, steroid hormones are thought to bind to cytoplasmic receptors and then regulate gene expression after they pass through the cell membrane. However, this mechanism does not show the potential changes of target cells. Some people through the glucocorticoid-induced neuronal membrane potential hyperpolarization research, demonstrated in recent years some researchers proposed steroid-mediated cell membrane potential changes. This new mechanism has important physiological implications for studying the nervous system, as rapid membrane effects are the basis of neuronal function. Experiments with guinea pig with visceral nerve celiac ganglion as a specimen, Kreb’s solution or cortisone and other solution surface perfusion of ganglia, intracellular microelectrode recording potential changes. The experimental results showed that: (1) The surface perfusion of ganglion neurons in guinea pigs can be performed by using 10 ~ (~) ~ 10 ~ (~) mol / L different concentrations of testosterone Induced the hyperpolarization and hyperpolarization with different amplitude corresponding to different membrane potential of nerve cells in less than 2 minutes. (2) Under the action of low Ca 2+ / Mg 2+ solution containing F, Membrane potential ultra-pole