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目的:探讨Ets-1和MMP-9在胎膜早破中的表达和意义。方法:采用免疫组织化学SP法在蛋白水平检测50例胎膜早破者(病例组)及45例无其他产科并发症及内外科合并症的足月择期剖宫产者(对照组)胎膜中Ets-1和MMP-9的表达。结果:①Ets-1蛋白在胎膜滋养层细胞核及胞浆中均有表达,以胞核表达为主;MMP-9主要表达于胎膜滋养层细胞浆中;②Ets-1和MMP-9在胎膜早破组滋养层中高表达,与对照组比较差异有统计学意义(P<0.05);③Ets-1和MMP-9在未足月胎膜早破组滋养层中高表达,但与足月胎膜早破组比较差异无统计学意义(P>0.05)。结论:Ets-1和MMP-9在人类胎膜上均有表达,且在胎膜早破中高表达;Ets-1可能通过上调MMP9的基因表达量使胎膜细胞外基质(ECM)的降解加速,致胎膜紧张度降低而引发胎膜早破。
Objective: To investigate the expression and significance of Ets-1 and MMP-9 in premature rupture of membranes. Methods: Immunohistochemical SP method was used to detect 50 cases of premature rupture of membranes (case group) and 45 cases of full-term elective cesarean section (control group) without other obstetric complications and surgical complications at the protein level In Ets-1 and MMP-9 expression. Results: ①Ets-1 protein was expressed in nucleus and cytoplasm of fetal trophoblast cells, mainly expressed in nucleus; MMP-9 protein was mainly expressed in cytoplasm of fetal trophoblast cells; ②Ets-1 and MMP-9 The expression of Ets-1 and MMP-9 in trophoblast cells of preterm premature rupture of membranes group was overexpressed, but was significantly higher than that in control group (P <0.05) Premature rupture of membranes group was no significant difference (P> 0.05). Conclusion: Ets-1 and MMP-9 are expressed in human fetal membranes and are highly expressed in premature rupture of membranes. Ets-1 may accelerate the degradation of fetal extracellular matrix (ECM) through the up-regulation of gene expression of MMP9 , Causing a decrease in fetal membrane tension caused by premature rupture of membranes.