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As baccatin III is an immediate diterpenoid precursor of taxol, the increase of baccatin III is benefi-cial to the biosynthesis of taxol. Addition of methyl jasmonic acid (MJ) enhances the activity of 10- deaceyle baccatin (DAB) III acetyl transferase which catalyzes the bioconversion from 10-DAB III to beccatin III. In this paper, the baccatin III content was increased by 174% by the addition of 100 μmol/L MJ in suspension cultures of Taxus cuspidate. Induction by MJ also increased the expression of a 49.0-kDa protein. This pa-per describes the cell free acetylation of 10-DAB III in crude extracts of enzyme from the suspension cul-tures of Taxus cuspidate. The reaction product was confirmed by high performance liquid chromatograph (HPLC). About 25.0% of the 10-DAB III was acetylized into baccatin III on the 4th day with 100 μmol/L MJ. The 10-DAB III acetyl transferase activity reached a peak on the 2nd day with 100 μmol/L MJ, with 54.7% of the 10-DAB III transformed into baccatin III. The baccatin III content increased with the increase of 10-DAB III acetyl transferase activity, although the biosynthesis was delayed by more than 24 h. The remarkable in-duction of MJ on baccatin III biosynthesis shows a promising way to increase the production of taxol.
As baccatin III is an immediate diterpenoid precursor of taxol, the increase of baccatin III is benefi-cial to the biosynthesis of taxol. Addition of methyl jasmonic acid (MJ) enhances the activity of 10-deaceyle baccatin (DAB) III acetyl transferase which catalyzes the bioconversion from 10-DAB III to beccatin III. In this paper, the baccatin III content was increased by 174% by the addition of 100 μmol / L MJ in suspension cultures of Taxus cuspidate. Induction by MJ also increased the expression of a 49.0 This pa-per describes the cell free acetylation of 10-DAB III in crude extracts of enzyme from the suspension cul tures of Taxus cuspidate. The reaction product was confirmed by high performance liquid chromatography (HPLC). About 25.0% of the 10-DAB III was acetylized into baccatin III on the 4th day with 100 μmol / L MJ. The 10-DAB III acetyl transferase activity reached a peak on the 2nd day with 100 μmol / L MJ, with 54.7% of the 10 -DAB III transformed into baccatin I. II. The baccatin III content increased with the increase of 10-DAB III acetyl transferase activity, although the biosynthesis was delayed by more than 24 h. The remarkable in-duction of MJ on baccatin III biosynthesis shows a promising way to increase the production of taxol.