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目的:探讨建立慢性充血性心力衰竭大鼠模型的方法。方法:采用腹主动脉缩窄法制备CHF大鼠模型。于右肾动脉分支处上方缩窄腹主动脉至0.6mm,同时设立假手术组及正常对照组。12周后行一般情况观察,超声心动图及血液动力学检测心脏结构和功能、计算心脏肥厚指数,HE及Masson染色检测心肌病理改变,综合评价CHF大鼠心功能。结果:与假手术及正常对照组比较,CHF模型组术后12周均出现进食减少、精神萎靡、少动、被毛无光;IVS、LVPW、LVM、LVEDD、LVESD、SV显著增加,EF、FS显著降低;HR显著增加;VSP、+dP/dtmax显著降低,T-dP/dtmax显著延长;LVEDP显著升高,-dP/dtmax绝对值显著降低;心脏湿重及心脏肥厚指数均增加;病理结果表明CHF模型组心肌细胞肥大、排列紊乱、心肌胶原纤维增多。结论:应用腹主动脉缩窄术可成功制备CHF大鼠模型。
Objective: To explore the method of establishing rat model of chronic congestive heart failure. Methods: Rat model of CHF was prepared by abdominal aorta constriction. At the junction of the right renal artery narrowed abdominal aorta to 0.6mm, at the same time set up sham operation group and normal control group. After 12 weeks, the general situation, the echocardiography and hemodynamics were used to detect the structure and function of the heart, the index of cardiac hypertrophy was calculated, the pathological changes of myocardium were detected by HE and Masson staining, and the cardiac function of CHF rats was evaluated comprehensively. Results: Compared with the sham operation group and the normal control group, the CHF group showed decreased food intake, low energy, low activity and no hair coat at 12 weeks after operation; the levels of IVS, LVPW, LVM, LVEDD, LVESD and SV were significantly increased (P <0.05), the HR increased significantly; VSP, + dP / dtmax decreased significantly, T-dP / dtmax increased significantly; LVEDP increased significantly, absolute value of -dP / dtmax decreased significantly; Cardiac wet weight and cardiac hypertrophy index increased; CHF model group myocardial cell hypertrophy, disordered arrangement, myocardial collagen fibers increased. Conclusion: CHF rat model can be successfully established by abdominal aortic constriction.