论文部分内容阅读
由芽孢杆菌Bacillus brevis 342-14的培养液中分离得一种新的肽类抗菌素brevi-stin(下简称BR)。 BR产生菌342-14的营养细胞是革兰氏阳性杆菌,其孢子易染色、椭园、端生到次端生。孢子囊膨大。在营养琼脂上半透明和奶油状的菌落呈环状、凸起和完整。将BR产生菌342-14接种于含葡萄糖1.0％,甘油0.5％,胨1.0％,麦芽浸出液0.5％及氯化钠0.3％(pH7.0)的培养基中,于28°振荡培养二天,再种入由葡萄糖1.0％,甘油0.25％,胨0.25％,黄豆粉1.0％
A novel peptide antibiotic brevi-stin (BR) was isolated from the culture of Bacillus brevis 342-14. The vegetative cells of BR-producing strain 342-14 are Gram-positive bacilli, the spores of which are easily stained, elliptical, and terminal to terminal. Sporocysts inflated. The translucent and creamy colonies on nutrient agar are ring-shaped, raised and intact. BR-producing bacteria 342-14 were inoculated into a medium containing glucose 1.0%, glycerol 0.5%, peptone 1.0%, malt extract 0.5% and sodium chloride 0.3% (pH 7.0) at 28 ° for two days with shaking Inoculated by glucose 1.0%, 0.25% glycerol, 0.25% peptone, soybean flour 1.0%