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目的:探讨选择性COX-2抑制剂Celecoxib对胃癌SGC-7901细胞增殖和凋亡的影响。方法:应用MTT法和FCM检测Celecoxib对胃癌SGC-7901细胞增殖、细胞凋亡、细胞周期和COX-2蛋白和bcl-2蛋白表达的影响。结果:Celecoxib呈时间、剂量依赖性抑制胃癌SGC-7901细胞生长,72h细胞最高存活率为48.7%,最低为20.5%;COX-2蛋白含量由26.73±0.06降至5.79±1.44,bcl-2蛋白含量由2.76±0.14降至0.78±0.05,COX-2蛋白和bcl-2蛋白表达的降低呈浓度依赖性;随着药物浓度的增高,细胞凋亡率由3.0%增加到33.0%;G0/G1期细胞比例增加到(87.29±1.34)%,S期和G2/M期细胞比例分别降低到(8.91±0.78)%、(3.80±0.55)%。结论:Celecoxib能够诱导胃癌SGC-7901细胞的凋亡,影响细胞周期的分布,从而有效地抑制胃癌SGC-7901细胞的增殖;Celecoxib诱导胃癌SGC-7901细胞的凋亡可能主要通过抑制COX-2的生物活性来实现,并与bcl-2蛋白表达的下调有关。
Objective: To investigate the effect of selective COX-2 inhibitor Celecoxib on proliferation and apoptosis of gastric cancer cell line SGC-7901. Methods: The effects of Celecoxib on the proliferation, apoptosis, cell cycle, the expression of COX-2 protein and bcl-2 protein in gastric cancer cell line SGC-7901 were detected by MTT and FCM. Results: Celecoxib could inhibit the growth of SGC-7901 cells in a dose-and time-dependent manner. The highest cell survival rate was 48.7% and the lowest was 20.5% at 72h. The COX-2 protein content was decreased from 26.73 ± 0.06 to 5.79 ± 1.44, while bcl- The content of COX-2 protein and bcl-2 protein decreased in a concentration-dependent manner from 2.76 ± 0.14 to 0.78 ± 0.05. The apoptosis rate increased from 3.0% to 33.0% with the increase of drug concentration. The G0 / G1 The percentage of senescent cells increased to (87.29 ± 1.34)%, while the proportion of cells in S phase and G2 / M phase decreased to (8.91 ± 0.78)% and (3.80 ± 0.55)%, respectively. Conclusion Celecoxib can induce the apoptosis of gastric cancer cell line SGC-7901 and the cell cycle distribution, and thus effectively inhibit the proliferation of gastric cancer cell line SGC-7901. Celecoxib induces the apoptosis of gastric cancer cell line SGC-7901 mainly through inhibiting the expression of COX-2 Biological activity to achieve and related to the down-regulation of bcl-2 protein expression.