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目的:建立尿中碘化物的液-液萃取毛细管气相色谱测定方法。方法:尿碘经衍生成易气化衍生物,通过有机溶剂萃取、离心,直接吸取有机层进样,经气相色谱分离,电子捕获检测器定量检测。结果:该法分离效果好,检出限为0.05μg/L,加标回收率为95.2%～98.6%,相对标准偏差(RSD)为1.0%～2.5%。结论:该方法简单,灵敏度高,可用于尿样中碘的测定。
Objective: To establish a method for the determination of iodine in urine by liquid-liquid extraction capillary gas chromatography. Methods: Urine iodine was derivatized into gas-permeable derivatives, which were extracted by organic solvent, centrifuged, and the organic layer was directly aspirated and separated by gas chromatography. Quantitative detection was performed by electron capture detector. Results: The separation efficiency was good, the detection limit was 0.05μg / L, the spiked recoveries were 95.2% -98.6%, and the relative standard deviations (RSDs) were 1.0% -2.5%. Conclusion: This method is simple, high sensitivity and can be used for the determination of iodine in urine samples.