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目的：探讨ＩＬ－３基因治疗脑胶质瘤的可能性。方法：以重组的复制缺陷型腺病毒作为基因转染的载体，在体外将鼠ＩＬ－３基因转染Ｇ４２２小鼠胶质母细胞瘤细胞，ＲＴ－ＰＣＲ检测ＩＬ－３基因转染的Ｇ４２２细胞中是否有转基因的ｍＲＮＡ转录；ＥＬＩＳＡ法检测细胞培养上清液中分泌的ＩＬ－３的水平；ＭＴＴ法检测细胞的体外增殖能力；ＩＬ－３基因转染Ｇ４２２细胞接种小鼠皮下，观察肿瘤的生长和小鼠的存活期，并检测小鼠脾细胞诱导的ＬＡＫ，ＣＴＬ的杀伤活性。结果：ＩＬ－３基因转染的Ｇ４２２细胞中有目的基因ｍＲＮＡ的转录，分泌高水平的ＩＬ－３。ＩＬ－３基因转染的Ｇ４２２细胞的体外增殖能力没有显著的改变。皮下接种后，肿瘤生长受抑制，荷瘤小鼠的存活期延长，脾细胞诱导的ＬＡＫ，ＣＴＬ杀伤活性增强。结论：ＩＬ－３基因转染的Ｇ４２２细胞的体内致瘤性降低，能激活宿主的抗肿瘤免疫反应
Objective: To investigate the possibility of IL-3 gene therapy for gliomas. Methods: The recombinant replication-defective adenovirus was used as a vector for gene transfection to transfect murine IL-3 gene into G422 mouse glioblastoma cells in vitro. RT-PCR was used to detect IL-4 transfected G422 cells. Whether there is transgene mRNA transcription; ELISA method to detect the level of IL-3 secreted in cell culture supernatant; MTT method to detect cell proliferation in vitro; IL-3 gene transfected G422 cells inoculated subcutaneously in mice to observe the tumor Growth and survival of mice, and detection of LAK and CTL killing activity induced by mouse spleen cells. RESULTS: The mRNA of the target gene was transfected into G422 cells transfected with IL-3 gene and secreted high levels of IL-3. There was no significant change in the in vitro proliferative capacity of G422 cells transfected with IL-3 gene. After subcutaneous inoculation, tumor growth was inhibited, tumor-bearing mice survived longer, and LAK and CTL killing activity induced by splenocytes increased. Conclusion: The tumorigenicity of G422 cells transfected with IL-3 gene is reduced in vivo, and can activate the host’s anti-tumor immune response.