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目的探讨CalpainI蛋白和细胞调亡在心房结构重构中的关系和作用。方法选择行换瓣手术的风湿性心脏病患者,在外科手术中取其左心房组织,用免疫印迹法测定calpainI蛋白含量;用TUNEL法检测左心房肌细胞凋亡,计算其凋亡指数(AI)。分析比较它们之间的关系。结果在慢性心房颤动(房颤)患者中,calpainI蛋白含量明显增加(344%±101.9%,P<0.001);AI明显升高(24.6%±9.1%,P<0.01)。CalpainI的蛋白含量和AI分别与左心房内径、房颤持续时间呈明显正相关(P<0.05～0.001);且CalapinI的蛋白含量与AI亦呈明显正相关(P<0.001)。结论慢性房颤时左心房组织中的calpainI蛋白含量和心肌细胞凋亡均增加,两者之间有协同作用,恶化心房的结构和功能,促进房颤的发生。
Objective To investigate the relationship between Calpain I protein and apoptosis in atrial remodeling. Methods Patients with rheumatic heart disease undergoing valve replacement surgery were enrolled. Left atrial tissue was removed during surgery. Calpain I protein content was determined by Western blotting. Apoptotic index of left atrial myocytes was measured by TUNEL. ). Analyze and compare the relationship between them. Results In the patients with chronic atrial fibrillation (AF), the calpain I protein content was significantly increased (344% ± 101.9%, P <0.001). The AI was significantly increased (24.6% ± 9.1%, P <0.01). Calpain I protein content and AI were significantly positively correlated with left atrial diameter and duration of atrial fibrillation, respectively (P <0.05-0.001). The protein level of CalapinI was positively correlated with AI (P <0.001). Conclusion The content of calpain I and the apoptosis of cardiomyocytes in the left atria are increased during chronic atrial fibrillation. The synergistic effect between the two mechanisms can ameliorate the structure and function of the atria and ameliorate the occurrence of atrial fibrillation.