【摘 要】
：
目的 研究抗癌痛方对通过胫骨内注射Walker 256细胞建立的癌症诱导性骨痛(CIBP)大鼠模型的抗伤害作用.方法 将96只4周龄SPF级雌性SD大鼠随机分成A组(16只)、B组(16只)、C组(64只).A组大鼠不做任何处理,B组大鼠左后腿胫骨注射无菌生理盐水15～20μl,C组大鼠左后腿胫骨注射肺癌细胞Walker 256建立CIBP模型.C组按照灌胃药物分为C1、C2、C3、C4组,每组16只.造模后14 d、15 d、16 d连续对C1组大鼠灌胃生理盐水(2 g/ml),C2组、C3组、C4组大
【机 构】
：
海口市人民医院,海南 海口570208
论文部分内容阅读
目的 研究抗癌痛方对通过胫骨内注射Walker 256细胞建立的癌症诱导性骨痛(CIBP)大鼠模型的抗伤害作用.方法 将96只4周龄SPF级雌性SD大鼠随机分成A组(16只)、B组(16只)、C组(64只).A组大鼠不做任何处理,B组大鼠左后腿胫骨注射无菌生理盐水15～20μl,C组大鼠左后腿胫骨注射肺癌细胞Walker 256建立CIBP模型.C组按照灌胃药物分为C1、C2、C3、C4组,每组16只.造模后14 d、15 d、16 d连续对C1组大鼠灌胃生理盐水(2 g/ml),C2组、C3组、C4组大鼠分别灌胃0.3 g/ml、0.6 g/ml、1.2 g/ml抗癌痛方.观察每次灌胃前、灌胃后30 min、灌胃后1 h大鼠的机械痛阈值.取B组大鼠10只,随机分为B1组和B2组两组,每组各5只,B1组不做任何处理,B2组给予抗癌痛方1.2 g/ml灌胃,连续3 d.取C1组大鼠10只,随机分为C1-1组和C1-2组两组,每组各5只,C1-1组不做任何处理,C1-2组给予抗癌痛方1.2 g/ml灌胃,连续3 d.采用Western Blot方法测量大鼠脊髓IL-1β、IL-6和TNF-α的蛋白质表达情况.结果 A组和B组大鼠各时点的机械痛阈值比较,差异均无统计学意义(P>0.05).C组大鼠术后7 d、10 d和14 d机械痛阈值较A组和B组大鼠同期明显降低,差异有统计学意义(P0.05).C组大鼠术后7d、10d和14d机械痛阈值较术前1d和术后1d、3d和5d明显降低,差异有统计学意义(P<0.05).与灌胃前比较,C2组、C3组和C4组大鼠术后14 d、15 d、16 d灌胃后30 min和灌胃后1 h的机械痛阈明显升高,差异有统计学意义(P<0.05).C2组、C3组和C4组大鼠术后14 d、15 d、16 d灌胃后30 min和灌胃后1 h的机械痛阈较C1组均明显升高,差异有统计学意义(P<0.05).C3组和C4组大鼠术后14 d、15 d、16 d灌胃后30 min和灌胃后1 h的机械痛阈较C2组明显升高,差异有统计学意义(P<0.05).与B1组比较,B2组的脊髓IL-1β、IL-6和TNF-α蛋白质表达明显降低,差异有统计学意义(P<0.05).与C1-1组比较,C1-2组脊髓IL-1β、IL-6和TNF-α蛋白质表达明显降低,差异有统计学意义(P<0.05).结论 抗癌痛方可通过抑制小胶质细胞和胶质细胞产生的促炎症性细胞因子的活化来改善CIBP.
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