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目的研究番石榴Psidium guajava叶的活性成分,为开发番石榴叶资源提供依据。方法以抗氧化活性为指标,运用多种柱色谱等现代分离手段对番石榴叶60%乙醇提取物进行活性追踪分离;根据理化性质和波谱学分析鉴定了化合物的结构,并评价了其抗氧化活性。结果分离得到12个化合物,分别鉴定为儿茶素(1)、鸢尾酚酮-2-O-β-D-吡喃葡萄糖苷(2)、山柰酚(3)、槲皮素(4)、槲皮素-3-O-α-L-呋喃阿拉伯糖苷(5)、槲皮素-3-O-β-D-吡喃阿拉伯糖苷(6)、槲皮素-3-O-β-D-吡喃半乳糖苷(7)、槲皮素-3-O-β-D-吡喃葡萄糖苷(8)、槲皮素-3-O-(6″-芥子酸)-β-D-吡喃半乳糖苷(番石柳叶苷A,9)、槲皮素-3-O-(6″-阿魏酸)-β-D-吡喃半乳糖苷(10)、顺式对香豆酸-4-O-β-D-吡喃葡萄糖苷(11)、去甲氧基荚果蕨素(12)。结论对分离得到的11个化合物进行抗氧化活性测试,其中化合物4、5、6显示较强活性,1、3、7、8显示中等强度活性。化合物2、10、11、12为首次从番石榴属植物中分离得到,化合物9为新化合物。
Objective To study the active components of the leaves of Psidium guajava and provide the basis for the development of the resources of Guava leaves. Methods The anti-oxidative activity was used as an index to separate and purify the 60% ethanol extract from the leaves of guava leaves using modern separation methods such as column chromatography. The chemical structure and chemical structure of the compounds were identified and their antioxidant activities were evaluated active. Results Twelve compounds were isolated and identified as catechin (1), irisone-2-O-β-D-glucopyranoside (2), kaempferol (3) , Quercetin -3-O-α-L-arabinofuranoside (5), quercetin-3-O-β-D-arabinopyranoside (6), quercetin-3-O- D-galactopyranoside (7), quercetin-3-O-β-D-glucopyranoside (8), quercetin -3-O- -Galactopyranoside A, 9, Quercetin-3-O- (6 "-feruic acid) -β-D-galactopyranoside (10) Coumadin-4-O-β-D-glucopyranoside (11), demethoxy podophyllin (12). Conclusion Eleven compounds were isolated and tested for antioxidant activity. Among them, compounds 4, 5 and 6 showed stronger activities and 1,3,7,8 showed moderate activity. Compounds 2, 10, 11 and 12 were isolated from guava plants for the first time, and compound 9 was a new compound.