大鼠膀胱癌的诱发和实验治疗研究

• 作 者: 范贤骏 谢建国 林培中 哈献文
• 机 构: 中国医学科学院肿瘤研究所,中国医学科学院肿瘤研究所,中国医学科学院肿瘤研究所,中国医学科学院肿瘤研究所
• 出 处: 实用癌症杂志
• 发表日期: 1987年01期
• 关键词: 大鼠膀胱 实验治疗 血卟啉 光敏剂 生化学 麻醉动物 炎细胞浸润 膀胱肿瘤 灌喂 上皮细胞

自从Ito等成功地应用亚硝基化合物BBN诱发出大鼠膀胱癌后,各种有关研究时有报导,但是国内报导极少。本组应用BBN成功地诱发了大鼠膀胱癌,并且以此模型进行血卟啉光敏治疗的实验研究,报告如下。 材 料 和 方 法 一、试剂和药品:N—丁基—N—(4—羟丁基)亚硝胺(BBN)系日本东京生化学研究所提供,贮于4℃冰箱。临用时以蒸馏水稀释成20％溶液。光敏剂HPD系北京制药工业研究所提供,贮于—20℃冰箱备用。

Since Ito et al. successfully used nitroso compound BBN to induce bladder cancer in rats, various studies have been reported, but there are few domestic reports. In this group, BBN was successfully used to induce bladder cancer in rats, and an experimental study of photosensitizing treatment of hematoporphyrin was performed using this model. The report is as follows. Materials and methods 1. Reagents and drugs: N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) was supplied by the Tokyo Institute of Biochemistry and stored in a refrigerator at 4°C. Diluted to 20% solution with distilled water upon use. Photosensitizer HPD is provided by the Beijing Institute of Pharmaceutical Industry and stored in a refrigerator at -20°C.