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目的比较微量取样与一次取样的优劣。方法36只家兔分为4组(每组9只),于死后12h取样:I组于一次取出所有VH;II组微量取样50μl,连续取15次;III组左眼一次取样。右眼微量重复取样。IV组双眼第1次微量取样,剩余一次取出。检测Ca2+、Cl-、K+、Na+和P等含量,数据统计分析。结果III组左右眼、(IV)组先后两次取样配对t检验无差异(P>0.05),各组微量取样样本的变异系数、双眼差较小。结论微量取样的操作影响较小、稳定性和重复性好,更适合法医学研究和应用。
Objective To compare the advantages and disadvantages of micro-sampling and one-time sampling. Methods Thirty-six rabbits were divided into four groups (n = 9). Samples were taken at 12h after death. Group I was removed all VHs at one time. Group II was subcutaneously sampled at 50μl for 15 consecutive times. Right eye trace repeated sampling. Group IV eyes first micro-sampling, the remaining time removed. Detection of Ca2 +, Cl-, K +, Na + and P content, data statistical analysis. Results There was no significant difference between paired t test (P> 0.05) between the two groups in the group III and the group IV. Conclusion The operation of micro-sampling has little effect, good stability and repeatability, and is more suitable for forensic research and application.