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目的： 探讨紫外线减毒弓形虫ＺＳ１ 株在小鼠体内的免疫保护性和细胞免疫反应。方法： 用波长为２５３７°Ａ 的紫外线照射弓形虫ＺＳ１株滋养体， 照射高度为５ ｃｍ ， 照射时间６０ ｍ ｉｎ。小鼠于免疫后４５ ｄ 用同株滋养体攻击感染， 攻击后４ ｄ 剖杀， 与单免疫组、单感染组及正常对照组小鼠比较其脾Ｔ淋巴细胞增殖反应及其亚群的变化。结果： 小鼠接种紫外线减毒弓形虫ＺＳ１ 株滋养体后能正常存活， 于接种后４９ ｄ 各组织未查见滋养体、包囊或假包囊； 免疫组攻击感染后存活时间较单感染组延长； 体外特异抗原刺激后， 可诱导免疫组及免疫攻击组强的脾Ｔ淋巴细胞增殖反应； 免疫攻击组ＣＤ４＋ Ｔ细胞明显下降， ＣＤ４＋ ／ＣＤ８＋ 比率倒置； 免疫组、免疫攻击组及感染组的ＮＫ细胞活性均明显增强。结论： 紫外线减毒弓形虫ＺＳ１株滋养体疫苗能够诱导免疫小鼠产生一定的抗攻击感染保护力， 其中ＣＤ８＋ Ｔ细胞和ＮＫ细胞可能发挥着重要作用。
Objective: To investigate the immunoprotective and cellular immune responses of attenuated Toxoplasma gondii ZS1 strain in mice. Methods: Toxoplasma gondii ZS1 trophozoites were irradiated with ultraviolet light with a wavelength of 2537 ° A at a height of 5 cm and an irradiation time of 60 mins. The mice were challenged with the trophozoites of the same strain 45 days after challenge, and dissected 4 days after the challenge. Compared with the mice in the single immunization group, the single infection group and the normal control group, the splenic T lymphocyte proliferation reaction and its subpopulation were changed . Results: The mice survived the trophozoites of attenuated Toxoplasma gondii ZS1, and no trophozoites, cysts or pseudocysts were found on the 49th day after inoculation. The survival time of the challenge group was significantly higher than that of the single infection group Prolonged. The stimulation of in vitro specific antigens induced strong proliferation of splenic T lymphocytes in immune group and immune attack group. CD4 + T cells in immune challenge group were significantly decreased and CD4 + / CD8 + ratio was upside down. Immunized group, immunized group and infected group NK cell activity were significantly enhanced. CONCLUSION: The attenuated Toxoplasma gondii ZS1 trophozoite vaccine can induce some protection against challenge infection in immunized mice, of which CD8 + T cells and NK cells may play an important role.