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以美国的哈克尼西棉细胞质雄性不育系ＤＥＳ－ＨＡＭＳ２７７的花药和一个陆地棉品种可育花药作为对照，对我国６个细胞质雄性不育棉花品系进行了小孢子发生的细胞学特征的研究和线粒体ＤＮＡ的ＲＡＰＤ分析。研究结果表明，不同不育系不但在雄性败育时期上而且在线粒体基因型上存在着明显的分化。中棉所１２Ａ和ＮＭ－１Ａ的败育时期与对照不育系ＤＥＳ－ＨＡＭＳ２７７的败育时期基本相同，是在造孢细胞增殖或小孢子母细胞形成时期；１０４－７Ａ、湘远４－Ａ、ＮＭ－２Ａ和ＮＭ－３Ａ的败育时期比ＤＥＳ－ＨＡＭＳ２７７迟，是在减数分裂时期。这一细胞形态学的划分与线粒体ＤＮＡ的ＲＡＰＤ标记归类基本一致。造孢细胞和小孢子母细胞退化导致雄性不育的主要细胞形态学特征是：染色体行为异常，胞内常含有２～４个微核，核仁穿壁很普遍，细胞质液泡化，认为绒毡层的退化与小孢子母细胞的败育密切相关。线粒体ＤＮＡ的ＲＡＰＤ分析还表明恢保关系相同的不育系之间可以存在着细胞质基因型的分化。
Cytological characteristics of microsporogenesis in 6 cytoplasmic male sterile cotton cultivars in China were studied with anther of Huckissimax cytoplasmic male sterility line DES-HAMS277 and a fertile anther of upland cotton variety as control And RAPD analysis of mitochondrial DNA. The results show that different male sterile lines not only in the male abortion period but also in mitochondrial genotypes there is a clear differentiation. The abortion period of CC-1A and NM-1A was basically the same as that of the control sterile line DES-HAMS277 during the period of sporulation or microspore blastocyst formation; 104-7A and Xiangyuan 4-A The aborting period of NM-2A and NM-3A was later than that of DES-HAMS277, which was at the stage of meiosis. This division of cell morphology is roughly consistent with the classification of mitochondrial DNA RAPD markers. Morphological characteristics of the main cells causing male sterility due to the degradation of the sporogenous cells and the microspore mother cells are as follows: chromosomal aberrations, intracellular often containing 2-4 micronuclei, nucleolus perforation is common, vacuolar cytoplasm, that the tapestry Degradation of layers is closely related to the abortion of microspore mother cells. RAPD analysis of mitochondrial DNA also showed that there may be cytoplasmic genotypic differentiation between CMS lines with the same conservation relationship.