论文部分内容阅读
目的研究不同剂量叶黄素对D-半乳糖(D-gal)小鼠氧化应激损伤的影响。方法44只昆明种小鼠随机分为D-gal模型对照组、叶黄素低剂量组、叶黄素高剂量组和正常对照组。D-gal120 mg/(kg.d)腹腔注射制作小鼠氧化应激模型,叶黄素低、高剂量组分别灌胃给予叶黄素10 mg/(kg.d)、40 mg/(kg.d),持续6周。检测小鼠肝组织活性氧(ROS)、丙二醛(MDA)、一氧化氮(NO)含量,超氧化物歧化酶(SOD)、总一氧化氮合酶(TNOS)、诱导型一氧化氮合酶(iNOS)、线粒体ATP酶(ATPase)活性和血清ALT、AST水平。结果叶黄素各剂量组小鼠肝组织MDA、ROS含量低于模型对照组;高剂量组SOD活性高于模型对照组,TNOS、iNOS活性低于模型组,肝线粒体Na+-K+-ATP酶活性高于模型组,差异均有统计学意义(P<0.05)。结论叶黄素可降低D-gal致氧化应激小鼠体内ROS和NOS活性,提高SOD和线粒体Na+-K+-ATP酶活性,从而缓解氧化应激损伤。
Objective To investigate the effects of different doses of lutein on oxidative stress in D-gal mice. Methods Totally 44 Kunming mice were randomly divided into D-gal model group, low-dose lutein group, high-dose lutein group and normal control group. The model of oxidative stress was established by intraperitoneal injection of D-gal 120 mg / (kg · d). Lutein (10 mg / (kg · d), 40 mg / kg / d) for 6 weeks. The levels of reactive oxygen species (ROS), malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), total nitric oxide synthase (TNOS), inducible nitric oxide (INOS), mitochondrial ATPase (ATPase) activity and serum ALT, AST levels. Results Compared with model control group, the content of MDA and ROS in liver tissue of lutein groups were lower than that of model control group. The activity of SOD in high dose group was higher than that in model control group. The activity of TNOS and iNOS in model group was lower than that in model group. Na + -K + -ATPase activity Higher than the model group, the difference was statistically significant (P <0.05). Conclusion Lutein can reduce ROS and NOS activity in oxidative stress-induced Mice induced by D-gal and increase the activity of SOD and mitochondrial Na + -K + -ATPase, thereby alleviating the oxidative stress injury.