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合成了新试剂４－（Ｈ－酸偶氮）１－苯基－３－甲基吡唑酮（ＨＰＭＰ），研究了其和铜的显色反应。在ｐＨ＝７的ＮＨ４Ａｃ缓冲介质中，ＣＴＭＡＢ存在下，ＨＰＭＰ与Ｃｕ（Ⅱ）生成２∶１紫色络合物，λｍａｘ＝５８０ｎｍ，ε＝５．６８×１０４Ｌ·ｍｏｌ－１·ｃｍ－１。铜含量在０～０．６ｍｇ／Ｌ内符合比耳定律。方法用于水样和生物样品中铜的测定，结果令人满意。
A new reagent 4- (H-acid azo) 1-phenyl-3-methylpyrazolone (HPMP) was synthesized and its color reaction with copper was studied. In NH4Ac buffer medium with pH = 7, HPMP and Cu (Ⅱ) generated purple complex with λmax = 580nm and ε = 5.68 × 104L · mol-1 · cm-1 in the presence of CTMAB. The copper content in 0 ~ 0.6mg / L in line with the Beer’s law. The method was applied to the determination of copper in water samples and biological samples with satisfactory results.