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目的检测癌睾丸抗原(CTA)MAGE-A3、AKAP-4在非小细胞肺癌(NSCLC)的表达及意义。方法应用间接酶免疫组化技术检测MAGE-A3、AKAP-4在45例肺癌组织中的表达情况,分析其与组织类型及淋巴结转移的相关性。结果 45例标本中MAGE-A3、AKAP-4在NSCLC中的敏感度分别为62.2%和73.3%,联合两种抗原可显著提高敏感性,为91.1%(P<0.05);MAGE-A3、AKAP-4在鳞癌中的表达率显著高于腺癌,与性别、年龄、淋巴结有无转移不相关。结论 MAGE-A3、AKAP-4是较好的NSCLC辅助诊断指标,联合应用两种蛋白可提高诊断敏感性。
Objective To detect the expression and significance of MAGE-A3 and AKAP-4 in non-small cell lung cancer (NSCLC). Methods The expression of MAGE-A3 and AKAP-4 in 45 lung cancer tissues was detected by indirect enzyme-linked immunosorbent assay (ELISA), and its correlation with tissue type and lymph node metastasis was analyzed. Results The sensitivity of MAGE-A3 and AKAP-4 in NSCLC were 62.2% and 73.3%, respectively. The combination of two antigens could significantly improve the sensitivity (91.1%, P <0.05) -4 in squamous cell carcinoma was significantly higher than that in adenocarcinoma, with or without gender, age, lymph node metastasis. Conclusion MAGE-A3 and AKAP-4 are good indicators of NSCLC diagnosis. Combined use of two proteins can improve the diagnostic sensitivity.