Detection of H pylori infection by ELISA and Western blot techniques and evaluation of anti CagA ser

• 作 者: (O)zlem Yilmaz Nazime (S)en Ahmet Ali Küpelio(g)lu (I)lkay (S)im(s)ek
• 机 构: Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Dokuz Eylül University In
• 出 处: 世界胃肠病学杂志（英文版）
• 发表日期: 2006年33期
• 关键词: H pylori Serum CagA Enzyme-linked immunoassay Western blot Serodiagnosis Dyspept

AIM: To detect H pylori infection and to evaluate the anti CagA seropositivity in adult Turkish dyspeptic patients. METHODS: We evaluated anti-H pylori IgA, IgG and anti-CagA antibodies using commercial enzyme-linked immunoassay (ELISA) and West blot in dyspeptic Turkish patients. H pylori status was determined by histology and rapid urease testing.RESULTS: Fifty-six patients were entered. Forty-eight (85.7%) out of the 56 patients were positive for H pylori.H pylori IgG seropositivity was 82.1%, IgA seropositivity 48.2%. CagA ELISA showed that IgG was positive in 50% and IgA in 30.4% of those with H pylori infections.West blot showed that IgG seropositivity was 80.4%and IgA seropositivity 33.9%. West blot detected IgG antibodies with reactivity to CagA in 50%, VacA in 62.5%, UreB in 87.5%, UreA in 80.4%, and OMP in 57.1%. None of the tests had a sensitivity and specificity above 80%.CONCLUSION: None of these commercial tests seems clinically useful for H pylori detection in adult dyspeptic patients, while West blot can give seropositivity and determine anti-CagA, VacA virulence factor status of Turkish dyspeptic patients in the Izmir region.