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细粒棘球蚴病在肯尼亚是一种重要的人兽共患病,其诊断主要靠手术和血清学方法。由于手术较危险,故以血清学诊断较好。血清学诊断主要用酶联免疫吸附试验(ELISA),以往所用的是Capron’s“Arc 5”抗原,用它来测定特殊的抗体。本文作者采用棘球蚴液中的一种脂蛋白(“抗原880”)作抗原,同时也用Capron’s“Arc 5”抗原来作ELISA,比较了两种抗原在诊断人棘球蚴病的效果。“抗原880”取自牛的棘球蚴液(HCF),将HCF以2000×g离心,并通过0.45μm的微孔滤膜过滤而净化,净化液再经PM 30超滤膜(截留点为30000道尔顿)超滤浓缩,浓缩液的蛋白质含量为240μg/ml。
Echinococcus granulosus is an important zoonosis in Kenya, and its diagnosis mainly depends on surgery and serological methods. Because surgery is more dangerous, so serodiagnosis is better. Serological diagnosis is mainly based on enzyme-linked immunosorbent assay (ELISA), which used to be Capron’s “Arc 5” antigen, which is used to determine specific antibodies. The authors used a lipoprotein (“antigen 880”) in Echinococcus granulosus as an antigen and also used Capron’s “Arc 5” antigen as an ELISA to compare the efficacy of the two antigens in the diagnosis of human hydatid disease. “Antigen 880” was taken from bovine hydatid cyst fluid (HCF), HCF was centrifuged at 2000 × g and filtered through a 0.45 μm Millipore filter, which was then passed through a PM 30 ultrafiltration membrane 30,000 daltons), the concentrate has a protein content of 240 [mu] g / ml.