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本文研究了在体内外照射条件下小鼠骨髓多向性造血干细胞(CFU-S)和粒系定向干细胞(CFU-C)的辐射敏感性。它们的D(?)值分别为103.4拉德和173.7拉德,反映了CFU-S和CFU-C是两类在性质上不全相同的干细胞群。在目前尚未建立起除啮齿类动物以外的多向性造血干细胞测定技术的情况下,我们应用体外琼脂培养技术,比较了在体外照射条件下狗与小鼠骨髓CFU-C的辐射敏感性,它们的D_0值分别为58.6拉德和190.2拉德,反映了两者在辐射敏感性上存在着明显的差异,为阐明不同种属动物的不同辐射耐受性提供了一个根据。联系到AET具有降低造血干细胞辐射敏感性以及提高动物的辐射耐受性的事实,进一步论证了在造血型放射病的发生和发展中,造血干细胞的损伤和修复是起着重要作用的。
In this study, the radiosensitivity of murine bone marrow-derived hematopoietic stem cells (CFU-S) and granulocyte-directed stem cells (CFU-C) was studied in vitro and in vivo. Their D (?) Values were 103.4 rads and 173.7 rads, respectively, reflecting that CFU-S and CFU-C are two groups of stem cells that are not all the same in nature. In the absence of multidirectional hematopoietic stem cell assay techniques other than rodents, we applied in vitro agar culture to compare the radiosensitivity of CFU-C to the bone marrow of mice and mice exposed to in vitro irradiation The values of D_0 were 58.6 rad and 190.2 rad, respectively, reflecting the significant difference in radiosensitivity between the two species, providing a basis for elucidating the different radiation tolerance of different species of animals. In connection with the fact that AET has the effect of reducing the radiation sensitivity of hematopoietic stem cells and improving the radiation tolerance of animals, it is further demonstrated that the injury and repair of hematopoietic stem cells play an important role in the occurrence and development of hematopoietic radiation sickness.