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目的 探讨神经生长因子 (nervegrowthfactor, NGF)及其神经生长因子受体 (nervegrowthfactorreceptor,NGFR)对先天性巨结肠(Hirschsprung’sdisease, HD)的调控作用。方法 采用RT PCR技术检测新鲜标本正常对照肠管、痉挛段、移行段和扩张段各 22例NGF和NGFRmRNA的表达,分析并统计其与临床病理特点之间的关系。结果 22例正常对照肠管 21例NGFmRNA表达阳性(95.5% ),20例NGFRmRNA表达阳性(90.9% );痉挛段 5例NGFmRNA表达阳性(22.7% ),6例NG FRmRNA表达阳性(27.3% );移行段 9例NGFmRNA表达阳性(49.9% ),7例NGFRmRNA表达阳性(31.8% );扩张段 18例NGFmRNA表达阳性 (81.8% ),19例NGFRmRNA表达阳性 (86.4% )。NGF和NGFRmRNA正常肠管与扩张段肠管中表达高度一致;痉挛段和移行段均为NGFmRNA和NGFRmRNA低表达。结论 NGF和NGFR与HD病理形态有相关性;HD的痉挛段和移行段肠管NGF及NGFR表达异常,提示NGF和NGFR基因可能与HD的发生有关。
Objective To investigate the regulatory effect of nerve growth factor (NGF) and its nerve growth factor receptor (NGFR) on Hirschsprung’s disease (HD). Methods RT-PCR was used to detect the expression of NGF and NGFR mRNA in 22 cases of intestinal tract, spastic segment, transitional segment and dilatation segment of fresh specimens. The relationship between them and clinicopathological features was analyzed. Results NGF mRNA was positive in 21 cases (95.5%) and NGF mRNA was positive in 20 cases (90.9%) in 22 cases of normal control intestine. The expression of NGF mRNA in 5 cases was 22.7% in spasmodic group and 6 cases was positive in NG FRmRNA (27.3%). NGF mRNA expression was positive in 49 cases (9.9%) in 9 cases and positive expression in 7 cases (31.8%) in 7 cases. NGF mRNA expression was positive in 81 cases (81.8%) and NGFR mRNA in 19 cases (86.4%). The expression of NGF and NGFR mRNA in normal intestine and dilatation segment was highly consistent. Both spasticity and transitional segment were low expression of NGF mRNA and NGFR mRNA. Conclusions There is a correlation between NGF and NGFR and HD pathology. Abnormal expressions of NGF and NGFR in intestine of spastic segment and transitional segment of HD suggest that NGF and NGFR genes may be related to the development of HD.