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目的:探讨大鼠体内雌激素对子宫动脉和冠状动脉的作用与两者的平滑肌细胞(SMC)表现型的关系。方法:HE染色观察子宫动脉和冠状动脉的的组织形态学结构。间接免疫荧光染色比较子宫动脉和冠状动脉SMC中平滑肌特异性肌动蛋白α-SM actin和非肌细胞型肌动蛋白β-NM actin平均荧光强度。Western blot检测子宫动脉和冠状动脉SMC的α-SM-actin和β-NM-actin含量,并检测β-雌二醇刺激下两者β-NM actin含量变化情况。结果:冠状动脉SMC以收缩型SMC为主,高表达α-SM actin;子宫动脉SMC以合成型SMC为主,高表达β-NMactin。在生理浓度β-雌二醇刺激下子宫动脉SMC的β-NM actin含量显著上升。结论:冠状动脉和子宫动脉的SMC表现型存在明显差异,前者显示收缩型SMC特点,后者接近合成型SMC。雌激素能够刺激合成型SMC的增殖。
Objective: To investigate the effect of estrogen on uterine artery and coronary artery in rats and their relationship with SMC phenotype. Methods: HE staining was used to observe the histological structure of uterine artery and coronary artery. The average fluorescence intensity of smooth muscle-specific actin-SM actin and non-myocyte actin β-NM actin in uterine artery and coronary artery SMC were compared by indirect immunofluorescence staining. The contents of α-SM-actin and β-NM-actin in uterine artery and coronary artery SMC were detected by Western blot, and the content of β-NM actin in both groups were detected by β-estradiol. Results: The SMC of coronary artery was predominately contracted SMC with high expression of α-SM actin. SMC of uterine artery was predominantly SMC with high expression of β-NMactin. The β-NM actin content of uterine artery SMC increased significantly in the physiological concentration ofβ-estradiol. Conclusion: The SMC phenotypes of coronary arteries and uterine arteries are obviously different. The former shows the characteristics of contracted SMCs, while the latter approaches the synthetic SMCs. Estrogen stimulates the proliferation of synthetic SMCs.