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目的了解医院感染铜绿假单胞菌不同血清型的耐药谱及分子流行病学特性。方法耐药谱选用Ｋ—Ｂ法和ＭＩＣ、Ａｌｋａｌｉｎｅｌｙｓｉｓ小量快速提取质粒，０．７％琼脂糖凝胶电泳获得质粒ＤＮＡ图谱。结果呼吸病区患者和氧气湿化瓶分离的ＰＡ血清４型均耐ＡＰ、ＣＺ、Ｔ和ＮＯＲ，烧伤科患者ＰＡ血清６型分别耐ＡＰ、ＣＺ、Ｔ和ＡＰ、ＣＢ、ＣＺ、ＣＸＴ、Ｔ，外科患者血清１１型均耐ＡＰ、ＣＢ、Ｐｉｐ、ＣＺ、ＣＦＰ、ＣＸＴ、ＣＡＺ、Ｔ、ＡＮ、ＧＭ和ＴＭ１１种抗生素，４株对照菌不同。质粒图谱显示：呼吸病区患者和氧气湿化瓶分离菌均有约４．０、１．８、１．７、Ｍｄａｌ３条质粒带；烧伤患者含有４．０、２．０、１．８Ｍｄａｌ３条和４．１Ｍｄａｌ１条质粒带；外科患者分离菌均有１条约３．９Ｍｄａｌ的质粒带；对照菌不同。结论采用联合分型技术对医院不同病区患者分离的不同血清型ＰＡ其同源性不同。治疗感染选用抗生素种类也不同，为临床医生在经验用药的基础上选用敏感有效抗生素起指导作用，并为医疗器械消毒效果观察提供依据。
Objective To understand the drug resistance spectrum and molecular epidemiological characteristics of different serotypes of Nosocomial Pseudomonas aeruginosa. Methods K-B method and MIC, Alkalinelysis were used to extract plasmids in small quantities and plasmid DNA was obtained by 0.7% agarose gel electrophoresis. Results PA serum type 4 was resistant to AP, CZ, T and NOR in respiratory patients and oxygen humidification bottles. PA serum type 6 in burn patients was resistant to AP, CZ, T and AP, CB, CZ, CXT, T The serum of type 11 patients were all resistant to AP, CB, Pip, CZ, CFP, CXT, CAZ, T, AN, GM and TM11 antibiotics. The plasmid map showed that there were about 4.0,1.8,1.7 Mdal plasmids in respiratory patients and Oxygen humidifier bottles, and the burn patients had 4.0, 2.0, 1.8Mdal3 And 4.1Mdal1 plasmid; Surgical patients isolated bacteria have a plasmid about 3.9Mdal; control bacteria different. Conclusion The different typing of PA with different serotypes in patients with different wards in hospitals is different with different typing methods. Selection of antibiotics for treatment of different types of infection is also different for clinicians on the basis of empirical medication with sensitive and effective antibiotics play a guiding role, and to provide evidence for the observation of disinfection of medical devices.