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目的:探讨虾青素对脐静脉内皮细胞(HUVEC)的抗氧化作用。方法:体外培养HUVEC,分为空白组、模型组(H2O22 mmol/L)、虾青素+H2O2组(0.1,1,10μmol/L虾青素预处理48 h后,加2 mmol/L H2O2处理1 h)。用MTT法检测细胞的存活率,DCFH-DA法检测细胞内ROS水平,JC-1法检测线粒体膜电位,Annexin V-FITC流式细胞术和DAPI法检测细胞凋亡,western blot法检测Caspase-3和p53蛋白表达。结果:与空白组相比,H2O2能明显造成HUVEC细胞的凋亡和坏死。虾青素可以降低H2O2引起的细胞死亡,减少活性氧的产生,使线粒体膜电位升高,凋亡率减少,Caspase-3和p53的表达下调。结论:虾青素对H2O2引起的HUVEC细胞死亡具有保护作用,其机制可能与保护线粒体功能有关。
Objective: To investigate the anti-oxidative effect of astaxanthin on human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were cultured in vitro and divided into blank group, model group (H2O2, mmol / L), astaxanthin plus H2O2 group (0.1, 1, 10 micromol / L astaxanthin for 48 h, 1 h). Cell viability was detected by MTT assay, ROS level was detected by DCFH-DA assay, mitochondrial membrane potential was measured by JC-1 assay, apoptosis was detected by Annexin V-FITC flow cytometry and DAPI method, Caspase- 3 and p53 protein expression. Results: Compared with the blank group, H2O2 could obviously induce the apoptosis and necrosis of HUVEC cells. Astaxanthin can reduce cell death caused by H2O2, reduce reactive oxygen species, increase mitochondrial membrane potential, decrease apoptosis rate, and down-regulate the expression of Caspase-3 and p53. Conclusion: Astaxanthin can protect HUVEC from H2O2-induced cell death, and its mechanism may be related to the protection of mitochondrial function.