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材料和方法:作者以实验室繁殖的8只黑猩猩为观察对象,每周采血2次检测丙氨酸转氨酶(ACT)活力,每周或双周肝活检作常规光学和电镜榆查,全部动物都有感染前及ALT升降各期的样本。接种材料为抗血友病物质——第八因子,此因子曾传染非甲非乙型肝炎给两例病人。接种方法通过黑猩猩传代进行,原始接种共用三批因子,一批直接给2只黑猩猩静脉注射,另二批混合后注射另1只动物。第二代接种用黑猩猩感染后的急性期血浆或肝匀浆,第三代接种用慢性期血浆。活检组织固定于4℃的4%甲醛及0.1M磷酸缓冲液中,薄切片用醋酸双氧铀枸缘酸铅染色。结果:全部动物接种后都出现急性肝炎的酶学和组织学改变,但有关甲肝病毒、乙肝病毒、巨细胞病毒及EB病毒感染或再感染的各种血清反应均为阴性。超微结构改变主要在胞浆,全部动物急性期肝细胞浆中都出现有特异的管型结构,其形态与Jackson等报告的一致,这些胞浆管型的出现及其数量与ALT
MATERIALS AND METHODS: A total of 8 chimpanzees were bred in our lab. Blood samples were collected twice a week for ACT activity. Weekly or biweekly liver biopsies were performed routine optics and electron microscopy. All animals Before infection and ALT rise and fall of the sample. The inoculation material is the anti-hemophilia substance - factor VIII, which has been transmitted to two patients with non-A, non-B hepatitis. The method of inoculation was carried out by passage of chimpanzees. The initial vaccination shared three batches of factors, one of which was given intravenously to two chimpanzees and the other two animals were injected after mixing. The second generation inoculated with chimpanzees infected with acute phase plasma or liver homogenate, the third generation of vaccinated with chronic phase plasma. Biopsies were fixed in 4% formaldehyde and 0.1 M phosphate buffer at 4 ° C and thin sections were stained with uranyl acetate-citrate lead. RESULTS: Enzymatic and histological changes of acute hepatitis occurred after inoculation in all animals, but all seroprevalences of hepatitis A virus, hepatitis B virus, cytomegalovirus and Epstein-Barr virus infection or re-infection were negative. Ultrastructural changes mainly in the cytoplasm, acute hepatocyte cytoplasm in all animals have a special tubular structure, the morphology and Jackson and other reports consistent with the appearance of these cytoplasmic tube type and its number and ALT