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目的：建立快速测定细胞色素Ｐ４５０ＣＹＰ１Ａ２酶活性的高压液相色谱方法．方法：取３００μＬ血浆样品，用β羟乙基茶碱作内标，经５ｍＬ氯仿／异丙醇（９∶１）萃取处理后，用００５％的乙酸、乙腈和甲醇作为基本流动相，采用梯度洗脱程序在ＯＤＳ柱上分离待测组分，紫外检测波长２８２ｎｍ．结果：无内源性物质干扰测定．次黄嘌呤、内标和咖啡因快速基线分离，三者的保留时间均小于１３分钟．次黄嘌呤和咖啡因的检测下限均为０１μｍｏｌ·Ｌ－１，线性范围分别为１－１００μｍｏｌ·Ｌ－１和１－２００μｍｏｌ·Ｌ－１，相关系数分别为０９９９９和０９９８７，变异系数分别小于６％和１０％．两者的平均相对回收率为９６％－１０８％．结论：本方法快速、灵敏，可用于人群ＣＹＰ１Ａ２酶活性研究．
Objective: To establish a rapid HPLC method for the determination of cytochrome P450CYP1A2 activity. Methods: 300μL plasma samples were extracted with βhydroxyethyl theophylline as internal standard and extracted with 5mL chloroform / isopropanol (9:1). After using 005% acetic acid, acetonitrile and methanol as the basic mobile phase , The gradient elution program was used to separate the components under test on the ODS column. UV detection wavelength was 282nm. Results: No endogenous substance interference determination. Hypoxanthine, internal standard and caffeine rapid baseline separation, the three retention time is less than 13 minutes. The lower limit of detection of hypoxanthine and caffeine were 01μmol·L-1, linear range was 1-100μmol·L-1 and 1-200μmol·L-1, the correlation coefficients were 09999 and 09987 , The coefficient of variation was less than 6% and 10% respectively. The average relative recovery between the two was 96% -108%. Conclusion: The method is rapid and sensitive and can be used to study CYP1A2 enzyme activity in human population.