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以不结球白菜(Brassica campestris L.ssp.chinensis Makino)子叶为外植体,考察壳寡糖对不结球白菜子叶离体培养再生体系的影响。在添加外源激素6-BA和NAA的条件下,比较了不同浓度(0.5、1.0、2.0和10.0 mg·L-1)壳寡糖对不结球白菜子叶形成愈伤组织、再生芽和再生不定根的影响。实验结果表明,低浓度的壳寡糖能促进子叶形成愈伤组织、再生芽。壳寡糖促进子叶形成愈伤组织和再生芽的最适浓度为0.5 mg·L-1,与其他浓度壳寡糖处理组相比,该浓度壳寡糖促进了子叶愈伤组织的形成,使出愈率达到92%。此外,该浓度壳寡糖能提高子叶的芽再生频率,使再生率达到80%,同时再生芽长度、叶绿素含量及外植体总鲜重达到最大,均显著高于对照组。然而,壳寡糖对再生芽生根有抑制作用,形成的不定根数目、平均根长和最长根长度均小于对照组。
The cotyledons of Brassica campestris L.ssp.chinensis Makino were used as explants to investigate the effect of chitooligosaccharides on the regeneration system of non-heading Chinese cabbage cotyledons. Under the conditions of adding exogenous hormones 6-BA and NAA, chitooligosaccharides from non-heading Chinese cabbage at different concentrations (0.5, 1.0, 2.0 and 10.0 mg · L-1) were compared to form callus, regenerated shoots and regeneration Effects of adventitious roots. The experimental results show that low concentrations of chitooligosaccharides can promote cotyledon callus formation, regeneration buds. The optimal concentration of chitooligosaccharides to promote cotyledon callus formation and regeneration buds was 0.5 mg · L-1. Compared with other chitooligosaccharides-treated chitooligosaccharides groups, the chitooligosaccharides promoted cotyledon callus formation, The cure rate reached 92%. In addition, chitooligosaccharides increased the regeneration frequency of cotyledons, the regeneration rate reached 80%, and the length of regenerated buds, chlorophyll content and total fresh weight of explants reached the maximum, which were significantly higher than that of the control group. However, chitooligosaccharides inhibited rooting of regenerative buds, and the number of adventitious roots, the average root length and the longest root length were all smaller than those of the control group.