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目的 :检测原发性肝细胞癌 (PHC)患者外周血单个核细胞表达 AFP m RNA情况和血清肿瘤特异性生长因子 (TSGF)的含量及探讨其临床意义。方法 :采用逆转录聚合酶链反应 (RT- PCR)检测 86例 PHC患者外周血 AFP m RNA,同时采用 TS-GF试剂盒检测患者血清 TSGF含量。结果 :PHC患者外周血 AFP m RNA阳性率为 5 3 .5 % (46/86) ,血清 TSGF阳性率为80 .2 % (69/86) ,AFP m RNA阳性率与 TSGF含量呈显著正相关 (r =0 .6415 ,P <0 .0 5 )。外周血 AFP m RNA阳性率、血清TSGF含量及阳性率均与肿瘤分期、癌灶大小及肝癌肝外转移显著正相关 (均为 P <0 .0 5 )。结论 :PHC患者外周血 AFP m R-NA阳性率与血清 TSGF水平呈显著正相关 ,两者均与肿瘤分期、癌灶大小及癌转移呈显著正相关。检测外周血 AFP m RNA和血清 TSGF含量对判断肝癌细胞发生肝内外转移的风险可能有较重要的参考价值
Objective: To detect the expression of AFP m RNA and serum tumor specific growth factor (TSGF) in peripheral blood mononuclear cells of patients with primary hepatocellular carcinoma (PHC) and to explore its clinical significance. Methods: Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect AFP m RNA in peripheral blood of 86 patients with PHC. At the same time, serum TSGF level was detected by TS-GF kit. Results: The positive rate of AFP m RNA in peripheral blood of PHC patients was 53.5% (46/86), the positive rate of serum TSGF was 80.2% (69/86), the positive rate of AFP m RNA was positively correlated with the level of TSGF (r = 0.6415, P <0.05). The positive rate of AFP m RNA in peripheral blood, serum TSGF content and positive rate were positively correlated with tumor stage, tumor size and extrahepatic metastasis of liver cancer (all P <0.05). Conclusion: The positive rate of AFP m R-NA in peripheral blood of patients with PHC is positively correlated with the level of serum TSGF, both of which are positively correlated with the tumor stage, the size of cancer and the metastasis of cancer. Detection of AFP m RNA in peripheral blood and serum TSGF levels may have more important reference value for judging the risk of intrahepatic and extrahepatic metastasis of hepatoma cells