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目的 研究携带ｔｋ 基因ＥＢ病毒复制子表达载体对人肝癌细胞的转导、杀伤和旁观者效应。方法 设更昔洛韦（ＧＣＶ）组、阿昔洛韦（ＡＣＶ） 组和对照组共３ 组，脂质体包裹质粒转染人肝癌细胞后分别加入不同浓度药物；已转染基因的细胞与未转染的细胞不同比例混合，每孔分别加入ＧＣＶ。样本７２ 小时后ＭＴＴ比色法检测杀伤和旁观者效应。结果 ＧＣＶ 或ＡＣＶ 均在０ ．２μｍｏｌ／Ｌ浓度即可对转染ｔｋ 基因的肝癌细胞产生杀伤作用并依浓度呈梯度变化。ＧＣＶ 在转染／未转染肝癌细胞１∶５ 的混合比率时即可产生明显的旁观者效应。结论 ＥＢ 病毒复制子载体适合携带ｔｋ 自杀基因用于肝癌基因治疗。
Objective To investigate the transduction, killer and bystander effects of the EB virus replicon expression vector carrying the tk gene on human hepatoma cells. Methods A total of three groups of ganciclovir (GCV), acyclovir (ACV) and control groups were set up. The liposome-encapsulated plasmids were transfected with human hepatoma cells and added with different concentrations of drugs; the cells that had been transfected with the genes were Untransfected cells were mixed in different proportions and GCV was added to each well. 72 hours after the sample, MTT colorimetry was used to detect killer and bystander effects. Results Both GCV or ACV are at 0. The concentration of 2 μmol/L can kill the hepatoma cells transfected with tk gene and change in gradient according to the concentration. GCV produced a significant bystander effect at a 1:5 mixing ratio of transfected/untransfected hepatoma cells. Conclusion The EB virus replicon vector is suitable for carrying tk suicide gene for gene therapy of liver cancer.