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在体外培养的家兔气道平滑肌细胞（ＡＳＭＣ）上，观察肾上腺髓质素（ＡＭ）对内皮素（ＥＴ）促ＡＳＭＣ增殖的影响及丝裂素活化蛋白激酶（ＭＡＰＫ）活性的变化。以探讨ＡＭ对ＡＳＭＣ增殖的调控。结果显示１０－８ｍｏｌ／ＬＥＴ－１显著刺激ＡＳＭＣ３Ｈ－ＴｄＲ参入及ＭＡＰＫ激活（Ｐ＜０．０１）。ＡＭ（１３－５２）呈剂量依赖地抑制ＥＴ－１的上述作用（Ｐ＜０．０５，Ｐ＜０．０１）。单独应用ＡＭ（１３－５２）对ＡＳＭＣ３Ｈ－ＴｄＲ参入及ＭＡＰＫ活性无明显影响。表明ＡＭ（１３－５２）可抑制ＡＳＭＣ对ＥＴ－１的增殖反应，其机理可能涉及ＭＡＰＫ活性的抑制。
The effects of adrenomedullin (AM) on the proliferation of ASMC and the activity of mitogen-activated protein kinase (MAPK) were observed in airway smooth muscle cells (ASMCs) cultured in vitro. To investigate the AM regulation of ASMC proliferation. The results showed that 10-8mol / LET-1 significantly stimulated ASMC3H-TdR incorporation and MAPK activation (P <0.01). AM (13-52) inhibited the above effects of ET-1 in a dose-dependent manner (P <0.05, P <0.01). AM (13-52) alone had no significant effect on ASMC3H-TdR incorporation and MAPK activity. It is indicated that AM (13-52) can inhibit the proliferative response of ASMC to ET-1, and its mechanism may involve the inhibition of MAPK activity.