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月季RcHSP17.8为编码小分子热激蛋白基因,Southern检测其在月季基因组中为多拷贝存在。38℃/3h热激处理后,该基因在耐热月季品种‘曼海姆宫殿’(Schloss Mannieim)、‘彩虹’(Radio)、‘赌城’(Las Vegas)、‘梅郎随想曲’(Capricede Meiland)、‘小女孩’(Girl)中强表达,而在不耐热品种‘新十全’(Kordes Perfecta)、‘绯扇’(Hiogi)、‘莱茵黄金’(Pfalzer Gold)中弱表达或不表达,表明该基因与月季耐热性关系密切。为确定月季RcHSP17.8基因功能,将其转化E.coliBL21,结果表明重组菌株能正常诱导表达包含RcHSP17.8的融合蛋白,并因此提高了重组菌株对高温、低温、高盐、高pH、重金属、氧化等非生物胁迫的耐性,表明RcHSP17.8参与了上述非生物胁迫的响应。
RcHSP17.8 is a small molecule heat shock protein gene, which is detected by Southern blotting in multiple copies in the rose genome. After 38¡æ / 3h heat shock treatment, the gene was cultivated in the heat-tolerant Rose varieties-Schloss Mannieim, Radio, Las Vegas, Capricede Meiland and Girl but weakly expressed in the heat-tolerant Kordes Perfecta, Hiogi and Pfalzer Gold or No expression, indicating that the gene is closely related with the heat resistance of roses. In order to determine the function of RcHSP17.8 gene, the recombinant plasmid was transformed into E.coliBL21. The results showed that the recombinant strain could induce the expression of fusion protein containing RcHSP17.8 normally, and thus enhanced the effect of recombinant strain on the expression of RcHSP17.8 at high temperature, low temperature, high salt, high pH, heavy metals , Oxidative stress and other abiotic stress tolerance, indicating that RcHSP17.8 involved in the abiotic stress response.