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研究了3种不同结构的糖分子(双十八烷基甘油基-β-葡萄糖苷(DGG)、1,2-O-双十八烷基-3-O-β-D-麦芽三糖基-甘油醇(DMTG)、3,6,9,12-四氧杂-10-胆甾基-2-乙酰胺-2-脱氧-β-D-吡喃葡萄苷(DL3))对大肠杆菌(E.coli)识别能力的影响。利用比色法测定囊泡对Z.coli的识别能力,实验结果表明:检测E.coli 3种体系的灵敏度大小顺序为DL3/TCDA>DMTG/TCDA>DGG/TCDA;具有多糖结构和水溶性的一(CH_2CH_2O)_3—结构的糖脂会有利于检测。红外光谱的研究证明了糖脂分子插入了聚联乙炔结构。
Three kinds of sugar molecules (DGG, 1,2-O-dioctadecyl-3-O-β-D-maltotriosyl Glycerol (DMTG), 3,6,9,12-Tetraoxa-10-Cholest-2-acetamide-2-deoxy-β-D-glucopyranoside (DL3) E.coli) ability to recognize. The colorimetric method was used to determine the ability of vesicles to recognize Z.coli. The experimental results showed that the sensitivity of the three kinds of E.coli systems was DL3 / TCDA> DMTG / TCDA> DGG / TCDA. The polysaccharide structure and water solubility A (CH_2CH_2O) _3- structure of the glycolipid will be conducive to detection. Infrared spectroscopic studies have demonstrated the insertion of glycolipid molecules into the polyacetylene structure.