论文部分内容阅读
用棉花枯萎病菌培养的毒素滤液,只有耐高温、耐稀释的特点。100℃处理15分钟,萎蔫指数72.3;5%的稀释度尚能致萎,最适浸苗浓度15～25%,最高产毒期14～16天,pH5的稀释液效果最好,pH5低于5或大于7致萎力都有影响。硫酸锌能鈍化毒素滤液的活性,在0.1ppm～1.5ppm之间随浓度增加而致萎力漸降。致病力不同的培养滤液,其致萎指数及最高致萎百分率有明显差異。用15%毒素滤液浸苗48小时,对棉花不同品种致萎力的差異结果与田间抗性相近。另外,用不同品种的棉籽仁丙酮浸提液与0.2%兔血稀释液混合,在显微镜下观察血球凝集情况,结果证明,凝集力愈强則品种抗性愈强,与田间抗性表现亦近似。
Fusarium oxysporum fusarium wilt bacteria culture filtrate, only high temperature, resistant to dilution characteristics. 100 ℃ treatment for 15 minutes, wilting index 72.3; 5% dilution is still able to wilt, optimum seedling concentration 15 ~ 25%, the highest producing period of 14 to 16 days, pH5 the best dilution, pH5 below 5 or more than 7 wilted have an impact. Zinc sulphate can deactivate the activity of the toxin filtrate, with the concentration increasing between 0.1ppm and 1.5ppm, the wilting force decreases gradually. Pathogenicity of different culture filtrate, the withered index and the maximum percentage of withered were significantly different. Soaking seedlings with 15% toxin filtrate for 48 hours resulted in a similar result to field resistance in wilt tolerance of different varieties of cotton. In addition, different varieties of cottonseed kernel acetone extract and 0.2% rabbit blood diluent were mixed under a microscope to observe the situation of blood coagulation, the results show that the stronger the cohesion, the stronger the variety resistance, and field performance similar to .