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目的 研究磁导向下磁性药物载体脑定向分布 ,磁性药物载体对肝、肾及骨髓的毒副作用 ,为脑胶质瘤的磁导向化疗提供可靠的依据。方法 昆明小鼠 5 0只 ,将一定量的磁性药物载体混悬液通过尾静脉注入小鼠体内 ,头部一侧放置聚焦磁场 ,定期给药观察 3个月 ,定时取血检查红细胞、白细胞、血小板、谷丙转氨酶 (ALT)、非蛋白氮 (NPN) ,并设立对照组。结果 7、14d磁导向组红细胞为(935 .0± 79.2 )、(92 5 .0± 81.5 )万 /mm3 ,肝功能及肾功能的变化不大 ,非磁导向组 (MTX组 )红细胞为(5 70 .0± 82 .2 )、(5 0 5 .0± 81.2 )万 /mm3 ,肝功能及肾功能的变化较大 ,两者差异有非常显著性(P <0 .0 1)。结论 磁导向下磁性药物载体定向分布于大脑靶区 ,改变了MTX随血流分布的特性 ,MTX在大脑定向浓集 ,而在肝、肾及骨髓的蓄积明显减少 ,显著减轻了对肝、肾及骨髓的损害作用。
Objective To study the brain-oriented distribution of magnetic drug carriers under magnetic guidance and the toxic and side effects of magnetic drug carriers on liver, kidney and bone marrow, so as to provide a reliable basis for magnetotactic chemotherapy of gliomas. Methods A total of 50 Kunming mice were injected with a certain amount of magnetic drug carrier suspension through the caudal vein. The magnetic field was placed on the side of the head for 3 months. The blood samples were collected for erythrocytes, Platelets, alanine aminotransferase (ALT), non-protein nitrogen (NPN), and set up a control group. Results The percentage of erythrocytes in magnetic guiding group was (935.0 ± 79.2) and (92.5 ± 81.5) million / mm3 on 7 and 14 days, respectively. The changes of liver function and renal function were not obvious. The erythrocytes in MTX group were ( 5 70 .0 ± 82 .2) and (5 0 5 .0 ± 81. 2) million / mm3 respectively. The changes of liver function and renal function were quite different. There was a significant difference between the two groups (P <0.01). CONCLUSION Magnetic targeting magnetic drug carriers are oriented in the target area of the brain, changing the distribution of MTX with blood flow. MTX concentrates in the brain and decreases in the liver, kidney and bone marrow significantly, And bone marrow damage.