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目的 应用ELISA 竞争（cELISA）法检测残余牛血清白蛋白（ESA）。方法 以BSA免疫家兔，获得高滴度的抗BSA血清；以BSA包被酶标板作为固相抗原，以梯度稀释的BSA为游离抗原，二者竞争结合抗体，以游离抗原浓度对数作为横坐标，相应吸收值为纵坐标作标准曲线，由对应样品的吸收值，可以求得样品中BSA含量。结果 当检测200、50、20和5ng/ml样品时，其试验内变异系数分别为4．5％、7．8％、5．8％和 14％，试验间变异系数分别为3．4％、8．8％、3.3％和30％。本试验还证明人血清白蛋白与牛血清白蛋白的交叉反应较低。结论cELISA可初步用于疫苗中残余BSA含量的检测。
Objective To detect residual bovine serum albumin (ESA) by ELISA (cELISA). Methods BSA was used to immunize rabbits to obtain high titer of anti-BSA serum. BSA-coated ELISA plates were used as solid phase antigen and gradient-diluted BSA was used as free antigen. The two antibodies competed for binding to antibodies and the logarithm of free antigen concentrations Abscissa, the corresponding absorption value of the vertical axis for the standard curve, the corresponding sample absorption value, can be obtained in the sample BSA content. Results When the samples were tested at 200, 50, 20 and 5 ng / ml, the coefficients of variation were 4.5%, 7.8%, 5.8% and 14%, respectively. The coefficients of variation were 3.4% , 8.8%, 3.3% and 30% respectively. This experiment also proved that human serum albumin and bovine serum albumin cross-reaction is low. Conclusion cELISA can be used initially in the detection of residual BSA in vaccines.