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We studied the effect of Coulomb interaction on the isospin fractionation in the intermediate energy heavy ion collisions by using the isospin-dependent quantum molecular dynamics model. The calculated results show that Coulomb interaction induces the reduction of the isospin fractionation process with the evolutions of neutronproton ratio and mass of system. Because Coulomb interaction is repulsive for the proton, more binding protons become free, which produces the neutron-poor gas phase and neutron-rich liquid phase, compared to the neutronproton ratio of the system. The isospin fractionation degree is weakened by the Coulomb term.