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目的:观察帕金森病(PD)模型大鼠黑质致密部(SNc)免疫球蛋白G(IgG)及其mRNA的表达变化,探讨Ig G在PD发病中的作用。方法:单侧微量注射6-羟多巴胺(6-OHDA)制备PD大鼠模型。免疫组化法观察对照组大鼠双侧、模型组大鼠健侧及注射侧SNc酪氨酸羟化酶(TH)阳性神经元和IgG阳性细胞的数量变化,RTPCR法检测两组大鼠黑质TH mRNA、IgG mRNA的表达变化。结果:模型组大鼠注射侧SNc内TH阳性神经元的数量较健侧显著减少(P<0.05),两组大鼠SNc均可见IgG阳性细胞,但模型组大鼠注射侧SNc IgG阳性细胞的数量显著增加(P<0.05),染色较深。模型组大鼠注射侧TH mRNA表达明显低于健侧和对照组双侧,IgG mRNA表达明显高于健侧和对照组双侧。结论:PD大鼠黑质致密部细胞IgG蛋白及其mRNA表达均增加,表明IgG与PD的发生有关。
OBJECTIVE: To observe the expression of immunoglobulin G (IgG) and its mRNA in the substantia nigra pars compacta (SNc) of Parkinson’s disease (PD) model rats and to explore the role of Ig G in the pathogenesis of PD. Methods: PD rat model was made by unilateral microinjection of 6-hydroxydopamine (6-OHDA). The number of SNc tyrosine hydroxylase (TH) positive neurons and the number of IgG positive cells on the contralateral side and the contralateral side of bilateral rats in the control group were observed by immunohistochemistry. RTPCR Changes in the expression of TH mRNA and IgG mRNA. Results: The numbers of TH positive neurons in SNc of model group were significantly decreased (P <0.05). SNc IgG positive cells were observed in both groups, but SNc IgG positive cells The number increased significantly (P <0.05), deep staining. The expression of TH mRNA in the model group was significantly lower than that in the contralateral group and control group, and the expression of IgG mRNA was significantly higher than that in the contralateral group and the control group. CONCLUSION: The expression of IgG and its mRNA in substantia nigra dense part of PD rats are increased, indicating that IgG and PD are involved in the pathogenesis of PD.