论文部分内容阅读
[目的]研究Hg2+对小麦种子萌发和幼苗生长的影响。[方法]以郑州9023为供试材料,采用室内水培方法,研究不同浓度(0.025、0.050、0.100、0.200、0.300、0.400、0.500mmol/L)Hg2+溶液浸种处理后,对小麦发芽率及幼苗苗高、根长、苗鲜重及叶片、根和萌发种胚中过氧化物酶(POD)、淀粉酶活性等影响。[结果]低浓度Hg2+(≤0.100mmol/L)对小麦种子萌发、幼苗苗高、根长和苗鲜重的影响较小。在Hg2+浓度>0.100mmol/L时,对小麦种子萌发和幼苗的正常生长有明显抑制作用。低浓度Hg2+(≥0.025mmol/L)可诱导小麦幼苗叶片、根和萌发种胚中POD酶活性明显升高,而抑制淀粉酶活性使之明显的下降,并且这种影响随着Hg2+浓度的增大而增强。[结论]Hg2+胁迫可以改变小麦幼苗叶片、根和萌发种胚中POD和淀粉酶活性,从而影响其膜脂氧化正常代谢及生长所需的能量和底物的供应不足,最终抑制了幼苗的生长。
[Objective] The research aimed to study the effect of Hg2 + on the seed germination and seedling growth of wheat. [Method] With Zhengzhou 9023 as test material, indoor hydroponic method was used to study the germination rate and seedling growth of wheat with different concentrations (0.025,0.050,0.100,0.200,0.300,0.400,0.500 mmol / L) of Hg2 + Seedling height, root length, seedling weight and peroxidase (POD) and amylase activity in leaves, roots and germinating embryos. [Result] Low concentration of Hg2 + (≤0.100 mmol / L) had little effect on seed germination, seedling height, root length and seedling weight of wheat. When Hg2 + concentration> 0.100mmol / L, the wheat seed germination and the normal growth of seedlings were significantly inhibited. Low concentrations of Hg2 + (≥0.025mmol / L) could induce POD activity in leaves, roots and germinating embryos of wheat seedlings significantly increased, while the activity of inhibiting amylase decreased significantly, and the effect of Hg2 + Large and enhanced. [Conclusion] Hg2 + stress could change POD and amylase activities in leaves, roots and germinating embryos of wheat seedlings, thus affecting the normal energy metabolism and the energy needed for their growth and the supply of substrate, eventually inhibiting the growth of seedlings .